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Comparison of The Number of Macrophage in Full Thickness Wound Incision between Merremia mammosa Extract Treatment and NaCl in Male Wistar Rats Hidayat, Fajar Kurniawan; Elfiah, Ulfa; Sofiana, Kristianningrum Dian
Journal of Agromedicine and Medical Sciences Vol 1, No 1 (2015)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

Show Abstract | Download Original | Original Source | Check in Google Scholar

The incidence of diabetes mellitus in Indonesia continues to increase. There are some complications in the diabetic condition, one of which is diabetic ulcer. Diabetic ulcers in diabetes patient can increase the risk of amputation and expensive treatment costs, so the alternative treatmeant such as Merremia mammosa wich has antiinflamatory and antidiabetic is needed. This study aimed to determine the comparison of the number of macrophage in the incisional wound in hyperglycemic male wistar rats between treatment with Merremia mammosa extract and NaCl. The in vivo test was done by creating wound incision on the mice backs and treated with gentamycin ointment 5% in a positive control group, NaCl in a negative control group and Merremia mammosa extract in a dose of 100m), 200mg, 400mg in treatment group. The result obtained by counting the number of macrophage in histopatholgy examination. The result showed the number of macrophage were 0,36 cells/field of view in a negative control group, 0,52 cells/field of view in a positive control group, 0,48 cells/field of view in all Merremia mammosa treatment groups. The data analysis showed no significant difference with p-value of 0,729. In conclusion, there was no significant difference between the used of Merremia mammosa extract and NaCl on full thickness incisional wounds of hyperglycemic male wistar rats. Keywords: Diabetes Mellitus, Merremia mammosa extract, incisional wound

Correlation between Cholinesterase Levels and Blood Glucose Levels in Farmers Exposed to Organophosphate Pesticide in Desa Sukorambi Kabupaten Jember Sofiannisa Achmadila; Kristianningrum Dian Sofiana; Yudha Nurdian
Journal of Agromedicine and Medical Sciences Vol 5 No 3 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i3.9652

The use of organophosphate pesticides by farmers can cause long-term health risks, one of which is an increase in blood glucose levels. This study aims to determine whether there is a relationship between the levels of cholinesterase as an indicator of exposure to organophosphate pesticides and blood glucose of farmers in Sukorambi Village, Jember Regency. Secondary data in the form of general characteristics of the sample were obtained through interviews, while the primary data was obtained from examining the levels of cholinesterase and blood glucose levels of farmers at the Biochemical Laboratory of the Faculty of Medicine, Jember University. The research method is observational-analytic with cross-sectional study design. Based on the results of statistical analysis using the Spearman correlation test (p = 0.05), it was concluded that there was no significant relationship between the two variables (p = 0.0802).

Relationship of Cholinesterase Activity with Blood Glucose Levels Due to Pesticide Exposure to Farmers in Mlokorejo Village Muhammad Ryznar Faisal Nur Luqmani; Kristianningrum Dian Sofiana; Bagus Hermansyah
Journal of Agromedicine and Medical Sciences Vol 7 No 1 (2021)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v7i1.20576

Increase in blood glucose levels can be caused by the long-term use of pesticides by farmers. This study aims to determine whether there is a correlation between the activity of cholinesterase as an indicator of exposure to pesticides and blood glucose of farmers in Mlokorejo Village, Jember Regency. The research method is observational-analytic with a cross-sectional study design. Data in the form of general characteristics of the sample were obtained through interviews, while the data of the activity of cholinesterase and blood glucose levels of farmers were obtained at the Biochemical Laboratory of the Faculty of Medicine, Jember University. The sample size of this study is 30 samples. All of the samples are having an increased activity of cholinesterase. Most of the samples are having normal blood glucose levels. Based on the results of statistical analysis using the Spearman correlation test (a= 0.05), it was concluded that there was no significant correlation between the two variables (p = 0,191). Keywords: blood glucose, cholinesterase, pesticides

Analysis of Low–level Cadmium Exposure Effects on HUVECs (Human Umbilical Vein Endothelial Cells) Cell Viability and Morphology Kristianningrum Dian Sofiana; Provisia Marthalita Y.W.; Khotimah Husnul; M Aris Widodo
Journal of Agromedicine and Medical Sciences Vol 5 No 1 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i1.9126

Cadmium is a heavy metal that could be found in daily life. This metal has a toxicity, could contaminate the environment, and affect human health. The main aim of this research was to find the effect of low concentration Cadmium exposure in acute time toward HUVECs cell morphology and viability.In a True experimental research with in vitro model using HUVECs cell, HUVECs cell was divided into four groups. One control group (without CdCl2 induction) and three treatment groups with CdCl2 induction with various concentrations, 0,153 µg/L, 1,53 µg/L and 15,3 µg/L. The trial was repeated five times for each group. Cell morphology was observed with an inverted microscope. Cell viability was examined by MTT assay. Data were analyzed using Kruskal Wallis statistical test and continue with the Man Whitney test. Correlation test was using Spearman.Morphology of treatment group HUVECs cell induced by CdCl2 concentration 15,3 µg/L looked significantly different compared with control group (p<0.05). Cell viability on group HUVECs induced by CdCl2 15,3 µg/L significantly different compared with the control group. The correlation test resulted R= -0,665 with probability 0.001 which means the higher concentration of CdCl2 the lower the viability of cells. Cadmium in low concentration induces cell morphology change and reduce cell viability. Keywords: HUVEC, cadmium, cell morphology, cell viability.

Comparison of The Number of Macrophage in Full Thickness Wound Incision between Merremia mammosa Extract Treatment and NaCl in Male Wistar Rats Fajar Kurniawan Hidayat; Ulfa Elfiah; Kristianningrum Dian Sofiana
Journal of Agromedicine and Medical Sciences Vol 1 No 1 (2015)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

The incidence of diabetes mellitus in Indonesia continues to increase. There are some complications in the diabetic condition, one of which is diabetic ulcer. Diabetic ulcers in diabetes patient can increase the risk of amputation and expensive treatment costs, so the alternative treatmeant such as Merremia mammosa wich has antiinflamatory and antidiabetic is needed. This study aimed to determine the comparison of the number of macrophage in the incisional wound in hyperglycemic male wistar rats between treatment with Merremia mammosa extract and NaCl. The in vivo test was done by creating wound incision on the mice backs and treated with gentamycin ointment 5% in a positive control group, NaCl in a negative control group and Merremia mammosa extract in a dose of 100m), 200mg, 400mg in treatment group. The result obtained by counting the number of macrophage in histopatholgy examination. The result showed the number of macrophage were 0,36 cells/field of view in a negative control group, 0,52 cells/field of view in a positive control group, 0,48 cells/field of view in all Merremia mammosa treatment groups. The data analysis showed no significant difference with p-value of 0,729. In conclusion, there was no significant difference between the used of Merremia mammosa extract and NaCl on full thickness incisional wounds of hyperglycemic male wistar rats. Keywords: Diabetes Mellitus, Merremia mammosa extract, incisional wound

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